Histone 3 antibody (H3K27me2)

Catalog No. ABIN2668465

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K27me2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)

Purification: None

Immunogen: This Histone H3 dimethylLys27 antibody was raised against a peptide including dimethyllysine 27 of histone H3.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668465)

Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." in: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Herz, Morgan, Gao, Jackson, Rickels, Swanson, Florens, Washburn, Eissenberg, Shilatifard: "Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling." in: Science (New York, N.Y.), Vol. 345, Issue 6200, pp. 1065-70, (2014) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Boulay, Dubuissez, Van Rechem, Forget, Helin, Ayrault, Leprince: "Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins." in: The Journal of biological chemistry, Vol. 287, Issue 13, pp. 10509-24, (2012) (PubMed).

Lafos, Kroll, Hohenstatt, Thorpe, Clarenz, Schubert: "Dynamic regulation of H3K27 trimethylation during Arabidopsis differentiation." in: PLoS genetics, Vol. 7, Issue 4, pp. e1002040, (2011) (PubMed).

Qi, Sarkissian, Hu, Wang, Bhattacharjee, Gordon, Gonzales, Lan, Ongusaha, Huarte, Yaghi, Lim, Garcia, Brizuela, Zhao, Roberts, Shi: "Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development." in: Nature, Vol. 466, Issue 7305, pp. 503-7, (2010) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020