Histone 3 antibody (H3K56ac)

Catalog No. ABIN2668471

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K56ac

Reactivity: Human, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Purification: Affinity Purified

Immunogen: This Histone H3 acetylLys56 antibody was raised against peptide containing acetylLys56 of yeast histone H3.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668471)

Meisenberg, Ashour, El-Shafie, Liao, Hodgson, Pilborough, Khurram, Downs, Ward, El-Khamisy: "Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan." in: Nucleic acids research, Vol. 45, Issue 3, pp. 1159-1176, (2017) (PubMed).

Tomazou, Sheffield, Schmidl, Schuster, Schönegger, Datlinger, Kubicek, Bock, Kovar: "Epigenome mapping reveals distinct modes of gene regulation and widespread enhancer reprogramming by the oncogenic fusion protein EWS-FLI1." in: Cell reports, Vol. 10, Issue 7, pp. 1082-95, (2015) (PubMed).

Yin, Lan, Zhang, Zhu, Chen, Xu, Liu, Ye, Sun, Lu, Zhang: "LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16." in: Molecular and cellular biology, Vol. 34, Issue 2, pp. 158-79, (2013) (PubMed).

Sims, Wade: "Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin." in: Molecular biology of the cell, Vol. 22, Issue 17, pp. 3094-102, (2011) (PubMed).

Foster, Dovey, Lezina, Luo, Gant, Barlev, Bradley, Cowley: "Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability." in: Molecular and cellular biology, Vol. 30, Issue 20, pp. 4851-63, (2010) (PubMed).

Dovey, Foster, Cowley: "Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 18, pp. 8242-7, (2010) (PubMed).

Koch, Pillus: "The glucanosyltransferase Gas1 functions in transcriptional silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 27, pp. 11224-9, (2009) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020