CD15 antibody

Catalog No. ABIN2688983

Product Details anti-CD15 Antibody

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95 % of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype, clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence intensity and its binding can be blocked by clone HI98. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of CD15 (W6D3) reactivity on peripheral blood granulocytes analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-human CD15 - Purified - Clone W6D3 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: W6D3

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD15 antibody (ABIN2688983)

Lund-Johansen, Olweus, Horejsi, Skubitz, Thompson, Vilella, Symington: "Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3221-9, (1992) (PubMed).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 (FUT4 Products)

Synonyms: AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody