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CD15 antibody
FUT4
Reactivity: Human
FACS
Host: Mouse
Monoclonal
W6D3
unconjugated
Product Details anti-CD15 Antibody
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Target
See all CD15 (FUT4) Antibodies
CD15 (FUT4)
(Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
Reactivity
All reactivities for CD15 antibodies
Human
Host
All hosts for CD15 antibodies
Mouse
Clonality
All clonalities for CD15 antibodies
Monoclonal
Conjugate
All conjugates for CD15 antibodies
This CD15 antibody is un-conjugated
Application
All applications for CD15 antibodies
Flow Cytometry (FACS)
Brand
BD Pharmingen™
Characteristics
Reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95 % of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype, clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence intensity and its binding can be blocked by clone HI98. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of CD15 (W6D3) reactivity on peripheral blood granulocytes analyzed by flow cytometry BD Pharmingen™ Purified Mouse Anti-human CD15 - Purified - Clone W6D3 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Clone
W6D3
Isotype
IgG1 kappa
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Discover our top product FUT4 Primary Antibody
Alternatives
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anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 251-295) antibody
FUT4
Reactivity: Human, Mouse, Rat
FACS, WB, IHC (p), ELISA, IHC (fro), IF (p), IF (cc)
Host: Rabbit
Polyclonal
unconjugated
Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Concentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
References for anti-CD15 antibody (ABIN2688983)
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Lund-Johansen, Olweus, Horejsi, Skubitz, Thompson, Vilella, Symington : "Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). " in: Journal of immunology (Baltimore, Md. : 1950) , Vol. 148 , Issue 10 , pp. 3221-9 , (1992 ) (PubMed ).
Target Details for CD15
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Target
CD15 (FUT4)
(Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
Alternative Name
CD15 (FUT4 Products )
Synonyms
AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody
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