CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN2689224, Supplier: Log in to see
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Reactivity
Mouse (Murine)
1283
600
250
69
61
41
32
30
28
26
25
25
22
21
18
18
15
12
11
10
10
9
8
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5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rat
1358
539
312
13
4
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
253
222
155
132
46
45
43
37
34
33
30
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
10
9
8
8
8
8
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4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
1703
545
473
300
285
272
217
165
91
73
49
39
33
26
21
19
18
14
13
11
11
9
7
7
6
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Mouse Thymocytes (BALB/c)
Clone RM4
Isotype IgG2a, kappa
Characteristics The RM4-5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells. CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone RM4-5 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others CD4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Synonyms: L3T4
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Martín, del Hoyo, Anjuère, Ruiz, Arias, Marín, Ardavín: "Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." in: Blood, Vol. 96, Issue 7, pp. 2511-9, 2000 (PubMed).

Shevach: "Regulatory T cells in autoimmmunity*." in: Annual review of immunology, Vol. 18, pp. 423-49, 2000 (PubMed).

Allman, Li, Hardy: "Commitment to the B lymphoid lineage occurs before DH-JH recombination." in: The Journal of experimental medicine, Vol. 189, Issue 4, pp. 735-40, 1999 (PubMed).

Bosselut, Zhang, Ashe, Kopacz, Samelson, Singer: "Association of the adaptor molecule LAT with CD4 and CD8 coreceptors identifies a new coreceptor function in T cell receptor signal transduction." in: The Journal of experimental medicine, Vol. 190, Issue 10, pp. 1517-26, 1999 (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, 1995 (PubMed).

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, 1995 (PubMed).

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994 (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, 1992 (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992 (PubMed).

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, 1991 (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, 1989 (PubMed).