SERPINB5 antibody

Catalog No. ABIN272300

Product Details anti-SERPINB5 Antibody

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINB5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Maspin protein. (region surrounding Thr118)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Alternative Name: SERPINB5 / Maspin (SERPINB5 Products)

Synonyms: 1110036M19Rik antibody, AI462524 antibody, AI646751 antibody, Maspin antibody, PI-5 antibody, Spi7 antibody, ovalbumin antibody, PI5 antibody, maspin antibody, Pi5 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody, serpin family B member 5 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antibody, Serpinb5 antibody, SERPINB5 antibody, serpinb5.S antibody

Background: Maspin is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells.Synonyms: PI-5, PI5, Peptidase inhibitor 5, Protease inhibitor 5, Serpin B5

Molecular Weight: approx. 42 kDa

Gene ID: 5268

NCBI Accession: NP_002630

UniProt: P36952

Pathways: p53 Signaling