CD4 antibody (Cy7,PE)

Catalog No. ABIN2739383

Product Details anti-CD4 Antibody (Cy7,PE)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to Cy7,PE

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Specificity: Antibody QS4120 recognizes the domain 1 region of the CD4 Molecule (1, 2).

Purification: Protein A purified

Immunogen: CD4+ transfectant/human CEM.

Type of Immunogen: Cells

Clone: QS4120

Isotype: IgG1

Application Details

Application Notes: Approved: Block, ELISA, Flo

Usage: Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of antihuman CD4/PE-Cy7 at a 1:50 dilution (5 μg/mL). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 41 % sub population of the cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-human CD4 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for CD4

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background: Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule

Gene ID: 920

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events