SERPINB5 antibody (Biotin)

Catalog No. ABIN465322

Product Details anti-SERPINB5 Antibody (Biotin)

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINB5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human Maspin

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Maspin.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hMaspin by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin. To detect hMaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Maspin (LS-B7484) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL sterile PBS containing 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Alternative Name: SERPINB5 / Maspin (SERPINB5 Products)

Synonyms: 1110036M19Rik antibody, AI462524 antibody, AI646751 antibody, Maspin antibody, PI-5 antibody, Spi7 antibody, ovalbumin antibody, PI5 antibody, maspin antibody, Pi5 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody, serpin family B member 5 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antibody, Serpinb5 antibody, SERPINB5 antibody, serpinb5.S antibody

Background: Name/Gene ID: SERPINB5

Synonyms: SERPINB5, Maspin, Peptidase inhibitor 5, PI5, Protease inhibitor 5 (maspin), Serpin B5, PI-5

Gene ID: 5268

UniProt: P36952

Pathways: p53 Signaling