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Recombinant Histone 3 antibody (acLys14)

H3 Reactivity: All Species WB, ELISA, ChIP, ICC, MA Host: Rabbit Monoclonal RM130 unconjugated Recombinant Antibody

Catalog No. ABIN2830998

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)

Antibody Type

Recombinant Antibody
Binding Specificity
56

47

44

41

40

36

35

35

34

34

34

32

29

29

28

27

26

25

23

22

22

22

20

18

18

17

16

16

16

16

16

16

15

14

14

13

13

12

12

12

11

11

11

10

10

10

10

9

9

8
acLys14
Reactivity
1369

939

860

59

42

41

41

39

39

35

35

29

26

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
All Species
Host
1183

242

8

4
Rabbit
Compatible Secondaries
Clonality
1109

327

1
Monoclonal
Conjugate
795

81

63

63

63

62

62

62

34

33

21

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
Application
1034

401

373

311

239

188

175

166

163

151

136

127

117

48

38

34

31

20

16

14

7

7

6

4

3

1

1

1
Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Multiplex Assay (MA)

Specificity

This antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), which is not affected by the modification of neighboring amino acids.

Cross-Reactivity (Details)

No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys14)

Clone

RM130

Isotype

IgG

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anti-Histone 3 (H3) (H3K4me) antibody
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anti-Histone 3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
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anti-Histone 3 (H3) (H3K36me3) antibody
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H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS with 1 % BSA and 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Expiry Date

12-24 months
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight

17 kDa

Gene ID

8350

UniProt

P68431