Estrogen Receptor alpha antibody (AA 2-185)

Catalog No. ABIN3023739

Product Details anti-Estrogen Receptor alpha Antibody

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Binding Specificity: AA 2-185

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.

Clone: NR3Ga-3

Isotype: IgG1 kappa

Application Details

Application Notes: Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

1. FFPE testing requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min and cooling at RT for 20 minutes.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IHC (Formalin-paraffin): 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ER antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor 1 (ESR1 Products)

Synonyms: LOC398734 antibody, AA420328 antibody, AU041214 antibody, ER-alpha antibody, ER[a] antibody, ERa antibody, ERalpha antibody, ESR antibody, Estr antibody, Estra antibody, Nr3a1 antibody, NR3A1 antibody, eralpha antibody, zfER[a] antibody, ER antibody, ESRA antibody, ESTRR antibody, Era antibody, akap12 antibody, cb27 antibody, esr1 antibody, fb72g12 antibody, id:ibd1202 antibody, sb:cb27 antibody, si:ch73-192g21.1 antibody, wu:fb72g12 antibody, wu:fc21c03 antibody, Esr antibody, RNESTROR antibody, ERALPHA antibody, er antibody, esr antibody, nr3a1 antibody, XER antibody, era antibody, esra antibody, ERbeta antibody, xesr-1 antibody, xlERalpha1 antibody, xlERalpha2 antibody, ESR1 antibody, estrogen receptor 1 L homeolog antibody, estrogen receptor 1 (alpha) antibody, estrogen receptor 1 antibody, A kinase (PRKA) anchor protein 12b antibody, esr1.L antibody, Esr1 antibody, ESR1 antibody, esr1 antibody, akap12b antibody

Background: Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Gene ID: 2099

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly