c-MYC antibody (C-Term)

Catalog No. ABIN3025307

This Mouse Monoclonal antibody specifically detects c-MYC in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for c-MYC antibody (C-Term) (ABIN3025307)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM237

Product Details anti-c-MYC Antibody

Binding Specificity: C-Term

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken

Purification: Protein G affinity chromatography

Immunogen: Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus was used as the immunogen for the Myc antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Myc antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc

Background: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect