beta-2 Microglobulin antibody
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- Target See all beta-2 Microglobulin (B2M) Antibodies
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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Reactivity
- Human, Non-Human Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This beta-2 Microglobulin antibody is un-conjugated
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Full length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody.
- Clone
- B2M-961
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product B2M Primary Antibody
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- Application Notes
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Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- Alternative Name
- beta-2 Microglobulin (B2M Products)
- Synonyms
- Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
- Background
- Recognizes a protein of 12 kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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