KIT antibody (AA 26-285)

Catalog No. ABIN3031690

Product Details anti-KIT Antibody

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Binding Specificity: AA 26-285

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 26-285) was used as the immunogen for this c-Kit antibody. Human protein shares 66% amino acid sequence identity with mouse protein.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the c-Kit antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the c-Kit antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: C-Kit (KIT Products)

Synonyms: C-Kit antibody, CD117 antibody, PBT antibody, SCFR antibody, Bs antibody, Fdc antibody, Gsfsco1 antibody, Gsfsco5 antibody, Gsfsow3 antibody, SCO1 antibody, SCO5 antibody, SOW3 antibody, Ssm antibody, Tr-kit antibody, W antibody, c-KIT antibody, c-kit antibody, MGF antibody, KIT antibody, C-KIT antibody, kit antibody, receptor antibody, sparse antibody, Xkl-1 antibody, kit-a antibody, kit-b antibody, kl1-A antibody, krk1 antibody, pbt antibody, scfr antibody, KIT proto-oncogene receptor tyrosine kinase antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antibody, KIT proto-oncogene receptor tyrosine kinase S homeolog antibody, KIT antibody, Kit antibody, kita antibody, kit antibody, kit.S antibody

Background: SCFR (Mast/stem cell growth factor receptor), also known as CD117, is a protein that in humans is encoded by the KIT gene. c-Kit was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. It is expressed on the surface of germ cells up to the pachytene stage. Signaling from the receptor, tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the c-Kit effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Gene ID: 3815

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity