NR3C2 antibody (AA 966-984)

Catalog No. ABIN3032062

Product Details anti-NR3C2 Antibody

Target: NR3C2 (Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2))

Binding Specificity: AA 966-984

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR3C2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids 966-984 (DQLPKVESGNAKPLYFHRK-human) were used as the immunogen for this Mineralocorticoid Receptor antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Mineralocorticoid Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Mineralocorticoid Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for NR3C2

Target: NR3C2 (Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2))

Alternative Name: Mineralocorticoid Receptor (NR3C2 Products)

Synonyms: mr antibody, si:ch211-189l17.1 antibody, LOC100302443 antibody, NR3C2 antibody, LOC443144 antibody, MLR antibody, MR antibody, MCR antibody, NR3C2VIT antibody, Mlr antibody, mlr antibody, nuclear receptor subfamily 3, group C, member 2 antibody, nuclear receptor subfamily 3 group C member 2 antibody, mineralocorticoid receptor antibody, nuclear receptor subfamily 3 group C member 2 L homeolog antibody, nr3c2 antibody, NR3C2 antibody, LOC443144 antibody, Nr3c2 antibody, nr3c2.L antibody

Background: NR3C2 (nuclear recepttor subfamily 3, group C, member 2) also known as MR (mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. It belongs to the nuclearreceptor family where the ligand diffuses into cells, interacts with the MR and results in a signal transduction affecting specific gene expression in the nucleus. MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system(hippocampus), brown adipose tissue and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports(mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body. The protein is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol. In intact animals, the MR is protected from glucocorticoids by co-localization of an enzyme, 11beta-hydroxysteroid dehydrogenase type 2(11beta-HSD2), that converts cortisol to inactive cortisone. It also responds to some progestins. Spironolactone and eplerenone are MR antagonists. Activation of the mineralocorticoid receptor, upon the binding of its ligand aldosterone, results in its translocation to the cell nucleus, homodimerization and binding to hormone response elements present in the promoter of some genes. This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes.

UniProt: P08235

Pathways: ACE Inhibitor Pathway, Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway