XRCC5 antibody (AA 462-475)

Catalog No. ABIN337306

Product Details anti-XRCC5 Antibody

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Binding Specificity: AA 462-475

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This XRCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human XRCC5 / Ku80.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SLAKKDEKTDTLED from an internal region of human XRCC5 / Ku80 (NP_066964.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Alternative Name: XRCC5 / Ku80 (XRCC5 Products)

Synonyms: AI314015 antibody, Ku80 antibody, Ku86 antibody, Kup80 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, NFIV antibody, xrcc5-a antibody, XRCC5 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog antibody, XRCC5 antibody, Xrcc5 antibody, xrcc5.L antibody

Background: Name/Gene ID: XRCC5

Synonyms: XRCC5, 86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP-1, Ku autoantigen, 80  kDa, NFIV, KARP1, Ku86, KUB2, TLAA, Nuclear factor IV, KU80, Thyroid-lupus autoantigen

Gene ID: 7520

NCBI Accession: NP_066964

UniProt: P13010

Pathways: DNA Damage Repair