Collagen IV antibody
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- Target See all Collagen IV (COL4) Antibodies
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Collagen IV antibody is un-conjugated
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody reacts with Human Type IV Collagen.
- Characteristics
- Synonyms: COL4A1
- Purification
- Purified
- Clone
- 2F11
- Isotype
- IgG1
- Top Product
- Discover our top product COL4 Primary Antibody
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- Application Notes
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ELISA: 1/2,000-1/8,000. Immunohistochemistry: < / = 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Alternative Name
- Collagen Type IV (COL4 Products)
- Synonyms
- collagen, type IV antibody, Let-2 antibody
- Background
- Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1
- Gene ID
- 1282
- UniProt
- P02462
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