DIO2
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Peptide ELISA: Detection limit: 1/128000. Western Blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart andThyroid lysates after 0.05 μg/mL DIO2 antibody staining. Immunohistochemistry: 2-3 μg/mL. Cryo-sectioned Mouse Brain (Hippocampus) showsstaining in the granular layer of the dentate gyrus. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Background
DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.Synonyms: DIOII, ITDI2, TXDI2, Type II iodothyronine deiodinase, Type-II 5'-deiodinase