CD45 antibody (APC)
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- Target See all CD45 (PTPRC) Antibodies
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivity
- Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD45 antibody is conjugated to APC
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Application
- Flow Cytometry (FACS)
- Specificity
- Chicken CD45, Mr 190-215 kDa
- Characteristics
- Mouse Anti-Chicken CD45-APC
- Purification
- Purified
- Immunogen
- Chicken bursal lymphocytes
- Clone
- LT40
- Isotype
- IgM
- Top Product
- Discover our top product PTPRC Primary Antibody
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with salmonella enteritidis." in: PLoS ONE, Vol. 7, Issue 2, pp. e32346, (2012) (PubMed).
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Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with salmonella enteritidis." in: PLoS ONE, Vol. 7, Issue 2, pp. e32346, (2012) (PubMed).
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- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Alternative Name
- CD45 (PTPRC Products)
- Synonyms
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- Background
- Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. 1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximately 90 % of thymocytes expressing fourfold higher levels of surface CD45 (CD45 hi ) than do the remaining 10 % (CD45 lo ) of thymocytes. The CD45 lo population contains exclusively thymocytes with the CD3 - CD4 - CD8 lo phenotype characteristic of the immediate precursors to the CD3 - CD4 + CD8 + thymic population which are CD45 hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4 - CD8 lo to CD4 + CD8 + and before the expression of CD3.
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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