c-MYC antibody (Biotin)

Catalog No. ABIN387707

There is 1 publication for this product available. The Mouse Monoclonal anti-c-MYC antibody is suitable to detect c-MYC in samples from Human. It has been validated for WB and ELISA.

Quick Overview for c-MYC antibody (Biotin) (ABIN387707)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 2G8D5

Product Details anti-c-MYC Antibody (Biotin)

Specificity: THE^TM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.

Characteristics: This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Purification: Protein A affinity column

Immunogen: A synthetic peptide EQKLISEEDL coupled - KLH

Isotype: IgG2a

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-c-MYC antibody (Biotin) (ABIN387707)

Armstrong, Xiao, Krill, Seroude, Dawson-Scully, Robertson: "Glial Hsp70 protects K+ homeostasis in the Drosophila brain during repetitive anoxic depolarization." in: PLoS ONE, Vol. 6, Issue 12, pp. e28994, (2011) (PubMed).

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: c-Myc

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect