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Vimentin antibody (AA 152-181)

Name: Vimentin antibody (AA 152-181)
SKU: ABIN389213
Price: 429 USD
Availability: InStock

VIM Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB15676 unconjugated

Western blot analysis of Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) in , cell line lysates (35 μg/lane). (Vimentin antibody (AA 152-181))

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (AA 152-181))

FACS

Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (ABIN389213 and ABIN2839368). (Vimentin antibody (AA 152-181))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Vimentin antibody (AA 152-181))

IHC (p)

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Vimentin antibody (AA 152-181))

IHC (p)

5 images

Catalog No. ABIN389213

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

16

16

12

11

8

8

7

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 152-181
Reactivity
324

125

124

60

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human
Host
186

123

9

6

4

4

2
Rabbit
Compatible Secondaries
Clonality
197

136
Polyclonal
Conjugate
191

19

13

13

10

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
273

126

98

95

94

58

46

40

39

28

23

18

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

B, Ha, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.

Clone

RB15676

Isotype

Ig Fraction

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VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

Application Notes

WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Molecular Weight

53652

Gene ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis