CACNA1D antibody (N-Term)

Catalog No. ABIN452403

Product Details anti-CACNA1D Antibody

Target: CACNA1D (Calcium Channel, Voltage-Dependent, L Type, alpha 1D Subunit (CACNA1D))

Binding Specificity: AA 859-875, N-Term

Reactivity: Rat, Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CACNA1D antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects Cav1.3 Ca2+ Channel at ~250 kDa. No cross-reactivity against Cav1.2.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Characteristics: Synonyms: CACH3, CACN4, CACNL1A2, CCHL1A2, Cav1.3, Voltage-dependent L-type calcium channelsubunit alpha-1D, Voltage-gated calcium channel subunit alpha, Calcium channel, L type,alpha-1 polypeptide, isoform 2

Purification: Protein G Chromatography

Immunogen: Fusion protein amino acids 859-875 (N-Terminal) of rat Cav1. 3 (also known as voltagedependent L-type calcium channel subunit alpha-1D, RAT brain class D and RBD)

Clone: S48A-9

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1-10 μg/mL (if results are off, try using the lysate without boiling). Immunoprecipitation. Immunocytochemistry: 0.1 - 1.0 μg/mL (Perox). Immunohistochemistry on frozen sections: 0.1 - 1.0 μg/mL (Perox). Immunflourescence: 1.0 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CACNA1D

Target: CACNA1D (Calcium Channel, Voltage-Dependent, L Type, alpha 1D Subunit (CACNA1D))

Alternative Name: CACNA1D (CACNA1D Products)

Synonyms: cacna1d antibody, cav1.3a antibody, isl antibody, si:dz136d19.3 antibody, si:dz223c6.1 antibody, ca(v)1.3 antibody, cav1.3 antibody, CACH3 antibody, CACN4 antibody, CACNL1A2 antibody, CCHL1A2 antibody, Cav1.3 antibody, SANDD antibody, 8430418G19Rik antibody, C79217 antibody, Cacnl1a2 antibody, Cchl1a antibody, Cchl1a2 antibody, D-LTCC antibody, calcium channel, voltage-dependent, L type, alpha 1D subunit, a antibody, calcium voltage-gated channel subunit alpha1 D antibody, calcium channel, voltage-dependent, L type, alpha 1D subunit antibody, calcium channel, voltage-dependent, L type, alpha 1D subunit L homeolog antibody, cacna1da antibody, CACNA1D antibody, cacna1d antibody, cacna1d.L antibody, Cacna1d antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage-gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), is a human gene. Cav1.3 subunits are primarily expressed in neurons and neuroendocine cells. Some studies suggest however that Cav1.3 is also found in the atria, and may figure prominently in atrial arrhythmias (3). Cav1.3 also carries the primary sensory receptors of the mammalian cochlea, and are also expressed in the electromotile outer hair cells (4).Synonyms: CACH3, CACN4, CACNL1A2, CCHL1A2, Calcium channel, Cav1.3, L type, Voltage-dependent L-type calcium channel subunit alpha-1D, Voltage-gated calcium channel subunit alpha, alpha-1 polypeptide, isoform 2

Gene ID: 29716

UniProt: P27732

Pathways: Sensory Perception of Sound