Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospho-(enol)pyruvate carboxylase from corn
Purification
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Immunogen
Phospho-(enol)pyruvate carboxylase isolated and purified from corn. Freund’s complete adjuvant is used in the first step of the immunization procedure.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
Restrictions
For Research Use only
Format
Lyophilized
Concentration
IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.8. No foreign proteins added.
Buffer
Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.