IGF1 antibody
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- Target See all IGF1 Antibodies
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This IGF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Mouse IGF-I
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Murine IGF-I.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IGF1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), Neut (12 - 14 μg/mL), WB
Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine IGF-I (200 ng/mL), a concentration of 12-14 μg/mL of this antibody is required. ELISA: To detect mIGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IGF-I (LS-C104336) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I. Western Blot: To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- Alternative Name
- IGF-I / IGF1 (IGF1 Products)
- Synonyms
- IGF-I antibody, IGF1A antibody, IGFI antibody, C730016P09Rik antibody, Igf-1 antibody, Igf-I antibody, Npt2B antibody, IGF-1 antibody, IGFIA antibody, IGF-IB antibody, igf1 antibody, IGF-1L antibody, IGF-1a antibody, IGF1 antibody, igf-1 antibody, igf1-A antibody, igf1.S antibody, xigf1 antibody, insulin like growth factor 1 antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I antibody, insulin like growth factor 1 L homeolog antibody, IGF1 antibody, Igf1 antibody, LOC100136741 antibody, igf1 antibody, LOC678666 antibody, igf1.L antibody, LOC104911603 antibody
- Background
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Name/Gene ID: IGF1
Family: Hormone
Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF - Gene ID
- 3479
- UniProt
- P05019
- Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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