Direct Elisa: To detect Human Maspin by Direct ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human Maspin. Sandwich Elisa: To detect Human Maspin by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedpolyclonal antibody, in conjunction with Affinity purified Anti-Human Maspin(ABIN487959 or ABIN487960) as a capture antibody, allows the detection of at least0.2-0.4 ng/well of recombinant Human Maspin. Western Blot: To detect Human Maspin by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human Maspin is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at-20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at-20 °C for longer.
Target
SERPINB5
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))
1110036M19Rik antibody, AI462524 antibody, AI646751 antibody, Maspin antibody, PI-5 antibody, Spi7 antibody, ovalbumin antibody, PI5 antibody, maspin antibody, Pi5 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody, serpin family B member 5 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antibody, Serpinb5 antibody, SERPINB5 antibody, serpinb5.S antibody
Background
MASPIN is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.Synonyms: PI-5, PI5, Peptidase inhibitor 5, Protease inhibitor 5, Serpin B5