Histone 3 (H3) (meLys9) antibody

Details for Product No. ABIN4889653, Supplier: Log in to see
Antigen
  • H-3
  • histocompatibility 3
  • H3
Epitope
meLys9
165
123
115
109
92
85
85
83
81
79
74
66
61
60
58
58
44
44
41
41
38
36
36
35
35
34
32
32
31
31
25
25
23
22
20
20
19
19
19
18
18
18
17
17
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16
16
16
16
16
15
15
15
14
13
11
10
10
10
9
9
9
9
9
8
7
7
7
7
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5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2362
1614
1032
574
94
75
74
74
71
69
60
49
37
35
34
32
31
23
15
10
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
2161
282
18
13
1
Clonality
Polyclonal
Conjugate
Un-conjugated
75
68
63
51
50
47
39
39
39
39
39
26
21
19
8
8
8
7
7
7
7
7
7
3
2
2
2
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
2225
1034
712
684
647
592
423
320
143
133
105
100
92
57
48
16
8
7
5
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen This Histone H3 monomethylLys9 antibody was raised against a peptide including monomethyllysine 9 of histone H3.
Purification None
Antigen
Alternative Name Histone H3
Background Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.
Molecular Weight 17 kDa
Gene ID 3020
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months
Supplier Images
Dot Blot (DB) image for anti-Histone 3 (H3) (meLys9) antibody (ABIN4889653) Histone H3 monomethyl Lys9 pAb tested by dot blot analysis. Dot blot analysis was use...
Immunofluorescence (IF) image for anti-Histone 3 (H3) (meLys9) antibody (ABIN4889653) Histone H3 monomethyl Lys9 pAb tested by immunofluorescence. Staining of HeLa cells w...
Western Blotting (WB) image for anti-Histone 3 (H3) (meLys9) antibody (ABIN4889653) Histone H3 monomethyl Lys9 pAb tested by Western blot. HeLa acid extract (10 µg per l...
Product cited in: Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, 2013 (PubMed).

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