ERCC1 antibody (AA 172-184)

Catalog No. ABIN490005

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Binding Specificity: AA 172-184

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ERCC1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine (100%) Horse (92%) Hamster, Pig, Opossum, Platypus, Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (C-QVDVKDPQQALKE) from internal region of human ERCC1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine (100%), Horse (92%), Hamster, Pig, Opossum, Platypus, Xenopus, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Synonyms: COFS4 antibody, RAD10 antibody, UV20 antibody, Ercc-1 antibody, ercc1 antibody, T12H1.18 antibody, T12H1_18 antibody, UV REPAIR DEFICIENT 7 antibody, UVR7 antibody, ERCC excision repair 1, endonuclease non-catalytic subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 1 antibody, DNA excision repair protein ERCC-1 antibody, nucleotide repair protein antibody, excision repair cross-complementation group 1 L homeolog antibody, excision repair cross-complementation group 1 antibody, ERCC1 antibody, Ercc1 antibody, CC1G_04546 antibody, CpipJ_CPIJ013903 antibody, PITG_13719 antibody, ercc1 antibody, ercc1.L antibody

Background: Name/Gene ID: ERCC1

Synonyms: ERCC1, COFS4, UV20, RAD10

Gene ID: 2067

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response