BAX antibody (AA 30-110)

Catalog No. ABIN4901051

The Mouse Monoclonal anti-BAX antibody has been validated for WB and IHC (p). It is suitable to detect BAX in samples from Human, Mouse, Rat and Chicken.

Quick Overview for BAX antibody (AA 30-110) (ABIN4901051)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6F11

Product Details anti-BAX Antibody

Binding Specificity: AA 30-110

Purpose: Bax is a protein encoded by the BAX gene which is approximately 21,1 kDa. Bax is localised to the mitochondrion membrane and cytoplasm. It is involved in PEDF induced signalling, apoptosis modulation and signalling and the TNFR1 pathway. This protein falls under the BCL2 protein family. It forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein interacts with, and increases the opening of, the mitochondrial voltage-dependent anion channel, which leads to the loss in membrane potential and the release of cytochrome c. Bax is expressed in a wide variety of tissues. Mutations in the BAX gene may result in colorectal cancer and leukaemia. STJ97725 was developed from clone 6F11 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous Bax protein.

Specificity: Bax Mouse Monoclonal Antibody (6F11) detects endogenous levels of BAX

Characteristics: Mouse Monoclonal to Bax.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from Bax

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Comment:

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response