ERCC1 antibody (AA 2-14)

Catalog No. ABIN490342

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Binding Specificity: AA 2-14

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human ERCC1. This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DPGKDKEGVPQPS-C from the N-terminus of human ERCC1 (NP_973730.1, NP_001974.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Synonyms: COFS4 antibody, RAD10 antibody, UV20 antibody, Ercc-1 antibody, ercc1 antibody, T12H1.18 antibody, T12H1_18 antibody, UV REPAIR DEFICIENT 7 antibody, UVR7 antibody, ERCC excision repair 1, endonuclease non-catalytic subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 1 antibody, DNA excision repair protein ERCC-1 antibody, nucleotide repair protein antibody, excision repair cross-complementation group 1 L homeolog antibody, excision repair cross-complementation group 1 antibody, ERCC1 antibody, Ercc1 antibody, CC1G_04546 antibody, CpipJ_CPIJ013903 antibody, PITG_13719 antibody, ercc1 antibody, ercc1.L antibody

Background: Name/Gene ID: ERCC1

Synonyms: ERCC1, COFS4, UV20, RAD10

Gene ID: 2067

NCBI Accession: NP_973730, NP_001974

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response