ERCC1 antibody (AA 179-191)

Catalog No. ABIN490349

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Binding Specificity: AA 179-191

Reactivity: Human, Mouse, Monkey, Rabbit, Zebrafish (Danio rerio), Pig, Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human ERCC1. This antibody is expected to recognise all three reported isoforms(NP_542937.1, NP_542938.1, NP_004383.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Elephant, Rabbit, Pig, Opossum, Chicken, Lizard, Zebrafish (100%) Stickleback, Medaka, Pufferfish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SPVENTPQNNECK from an internal region of human ERCC1 (NP_542937.1, NP_542938.1, NP_004383.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Elephant, Rabbit, Pig, Opossum, Chicken, Lizard, Zebrafish (100%), Stickleback, Medaka, Pufferfish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 75 and 52 kDa bands observed in Human Kidney lysates (calculated MW of 73.0 kDa according to NP_542937.1 and of 51.9 kDa according to NP_004383.2). Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Synonyms: COFS4 antibody, RAD10 antibody, UV20 antibody, Ercc-1 antibody, ercc1 antibody, T12H1.18 antibody, T12H1_18 antibody, UV REPAIR DEFICIENT 7 antibody, UVR7 antibody, ERCC excision repair 1, endonuclease non-catalytic subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 1 antibody, DNA excision repair protein ERCC-1 antibody, nucleotide repair protein antibody, excision repair cross-complementation group 1 L homeolog antibody, excision repair cross-complementation group 1 antibody, ERCC1 antibody, Ercc1 antibody, CC1G_04546 antibody, CpipJ_CPIJ013903 antibody, PITG_13719 antibody, ercc1 antibody, ercc1.L antibody

Background: Name/Gene ID: ERCC1

Synonyms: ERCC1, COFS4, UV20, RAD10

Gene ID: 2067

NCBI Accession: NP_542937, NP_542938, NP_004383

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response