Western Blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This HGF antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.01 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional heterodimeric polypeptide and an effector of cells expressing the Met tyrosine kinase receptor. It mediates the growth and scattering of various cell types, epithelial mesenchymal transition, the formation of tubules and lumens and promotes angiogenesis. The ligand-receptor pair has also shown to be uniquely involved in most human solid tumors and to participate in tumor development, invasion and metastasis.Synonyms: HPTA, Hepatopoeitin-A, Scatter factor