MUTYH antibody

Catalog No. ABIN498451

Product Details anti-MUTYH Antibody

Target: MUTYH (MutY Homolog (E. Coli) (MUTYH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUTYH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MYH protein. (region surrounding Tyr176)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immuofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MUTYH

Target: MUTYH (MutY Homolog (E. Coli) (MUTYH))

Alternative Name: MUTYH (MUTYH Products)

Synonyms: MUTYH antibody, MGC145569 antibody, CYP2C antibody, MYH antibody, 5730495A01Rik antibody, Mutyha antibody, Mutyhalpha antibody, Mutyhb antibody, Mutyhbeta antibody, Mutyhc antibody, Myh antibody, mutY DNA glycosylase antibody, mutY homolog (E. coli) antibody, A/G-specific adenine DNA glycosylase-like antibody, MUTYH antibody, mutyh antibody, LOC100373318 antibody, Mutyh antibody

Background: MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.Synonyms: A/G-specific adenine DNA glycosylase, MYH, MutY homolog

Molecular Weight: approx. 68 kDa

Gene ID: 4595

NCBI Accession: NP_001041636

UniProt: Q9UIF7

Pathways: DNA Damage Repair