FOXO1 antibody
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- Target See all FOXO1 Antibodies
- FOXO1 (Forkhead Box O1 (FOXO1))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FOXO1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of FKHR/FOXO1A protein. (region surrounding Ser262)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Purity
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product FOXO1 Primary Antibody
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- Application Notes
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FOXO1 (Forkhead Box O1 (FOXO1))
- Alternative Name
- FOXO1A (FOXO1 Products)
- Synonyms
- FKH1 antibody, FKHR antibody, FOXO1A antibody, FoxO1a.1 antibody, foxo1 antibody, zgc:153388 antibody, Fkhr antibody, Foxo1a antibody, AI876417 antibody, Afxh antibody, Fkhr1 antibody, fkh1 antibody, fkhr antibody, foxo1a antibody, xFoxO1 antibody, FoxO1A antibody, foxo1a.2 antibody, forkhead box O1 antibody, forkhead box O1 a antibody, forkhead box O1 L homeolog antibody, forkhead box O1 b antibody, FOXO1 antibody, foxo1a antibody, Foxo1 antibody, foxo1 antibody, foxo1.L antibody, foxo1b antibody
- Background
- FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is an ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2,13) and t(1,3) translocations, are commonly found in alveolar rhabdomyosarcomas.Synonyms: FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma
- Molecular Weight
- approx. 70 to 82 kDa
- Gene ID
- 2308
- NCBI Accession
- NP_002006
- UniProt
- Q12778
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling
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