Glutaminase antibody (C-Term)

Catalog No. ABIN5539364

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Binding Specificity: AA 516-545, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For IF starting dilution is: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS (GLS Products)

Synonyms: Glut antibody, RATGLUT antibody, gls antibody, si:dz87i4.2 antibody, wu:fa97e05 antibody, GA antibody, PAG antibody, AAD20 antibody, GAC antibody, GAM antibody, GLS1 antibody, KGA antibody, AI314027 antibody, 6330442B14 antibody, B230365M23Rik antibody, CG8772 antibody, CG8872 antibody, Dmel\\CG42708 antibody, Dmel_CG8772 antibody, BA3155 antibody, glutaminase antibody, glutaminase b antibody, Glutaminase antibody, glutaminase kidney isoform, mitochondrial antibody, Gls antibody, GLS antibody, gls antibody, glsb antibody, glsA2 antibody, glsA-2 antibody, Arnit_3113 antibody, Celal_2913 antibody, Celly_0289 antibody, Weevi_2101 antibody, Mesop_4683 antibody, LOC100069617 antibody

Background: Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Molecular Weight: 73 kDa

Gene ID: 2744

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect