PPP3CA antibody

Catalog No. ABIN126997

Product Details anti-PPP3CA Antibody

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP3CA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 267 of human Calcineurin A.

Application Details

Application Notes: Western Blot: 1-4 μg/mL. Immunoprecipitation: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PPP3CA

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Abstract: PPP3CA Products

Synonyms: CALN antibody, CALNA antibody, CALNA1 antibody, CCN1 antibody, CNA1 antibody, PPP2B antibody, 2900074D19Rik antibody, AI841391 antibody, AW413465 antibody, CN antibody, Caln antibody, Calna antibody, CnA antibody, Calna1 antibody, calcineurin antibody, caln antibody, calna antibody, calna1 antibody, ccn1 antibody, cna1 antibody, ppp2b antibody, fj46e08 antibody, si:dkeyp-79c2.2 antibody, wu:fj46e08 antibody, ppp3ca antibody, protein phosphatase 3 catalytic subunit alpha antibody, protein phosphatase 3, catalytic subunit, alpha isoform antibody, protein phosphatase 3, catalytic subunit, alpha isozyme antibody, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) antibody, protein phosphatase 3, catalytic subunit, alpha isozyme L homeolog antibody, serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody, PPP3CA antibody, Ppp3ca antibody, ppp3ca antibody, ppp3ca.L antibody, LOC100205420 antibody

Background: Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.Synonyms: CALNA, CAM-PRP catalytic subunit, CNA, Calmodulin-dependent calcineurin A subunit alpha isoform, PP2B catalytic subunit, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, protein phosphatase 2B catalytic subunit alpha

Gene ID: 5530

NCBI Accession: NP_000935

UniProt: Q08209

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling