Immunohistochemistry on paraffin sections (5.6 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1 7, NMDA1 3, KAI 2) which comprise ligand gated calcium channels. Metabotropic glutamate receptors are G protein coupled receptors that contain 7 membrane spanning domains and a large extracellular N terminal region. When activated, they can activate phopholipases or adenylylcyclase, depending on the neuron. The group I receptors mGluR1 and mGluR5 activate phospholipase C. By screening a human brain cDNA library with a rat mGluR1 alpha cDNA under low stringency conditions, Minakami et al. (1993) and Minakami et al. (1994) isolated human cDNAs encoding mGluR5. Minakami et al. (1993) identified a longer isoform of mGluR5 (mGluR5B).Synonyms: GPRC1E, MGLUR5, Metabotropic glutamate receptor 5