This antibody is specific for phosphorylated Human Plk-1 protein at the pThr210 residue. Cross reactivity with Plk-1 protein homologues from C.elgans and Honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Cross-Reactivity (Details)
Species reactivity (expected):Chimpanzee, Pig, Chicken, Mouse, Rat, Xenopus, Dog, Mosquito, Zebrafish, Starfish, Sea urchin and Fruit Fly. Species reactivity (tested):Human.
Purification
Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities
Immunogen
Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein
ELISA (1/3,000-1/12,000). Immunohistochemistry on Paraffin Sections (1/200-1/1,000). This antibody is positive byIHC on kidney, liver, cancer, thyroid and lymphocyte tissue. Western blot (1/200-1/2,000). Expect a band approximately 68 kDa in size correspondingto Plk-1. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1