Collagen IV antibody

Catalog No. ABIN5610391

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: Collagen IV is a protein encoded by the COL4A1 gene which is approximately 160,6 kDa. Collagen IV is secreted into the extracellular space. It is involved in collagen chain trimerization, the integrin pathway, ERK signalling and focal adhesion. It is the major structural component of glomerular basement membranes and forms a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. It specifically inhibits endothelial cell proliferation, migration and tube formation. Collagen IV is expressed in the nervous system, liver, lung, heart and kidney. Mutations in the COL4A1 gene may result in the tortuosity of retinal arteries. STJ98907 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous Collagen IV protein.

Specificity: The antibody detects endogenous Collagen IV protein.

Characteristics: Mouse Monoclonal to Collagen IV.

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: synthetic peptide derived from Collagen IV.

Application Details

Application Notes: IF 1:50-200
IHC-P 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Molecular Weight: 161kDa

UniProt: P02462