Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
This antibody detects endogenous level of AFX only when phosphorylated at serine 197.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.