Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C.
Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.