This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
Paxillin (Phospho-Tyr31) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin.
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions