Profilin antibody

Catalog No. ABIN5689875

Product Details anti-Profilin Antibody

Target: Profilin (PFN)

Reactivity: Human, Cow, Mouse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Profilin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Recognizes human, mouse, rat, pig, bovine and marsupial profilin.

Purification: Antigen affinity purified.

Immunogen: Human full-length profilin.

Application Details

Application Notes: ELISA: (direct or indirect: 1:2,000-1:5,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Liquid. In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for at least 1 year after receipt when stored at -20°C.

Target Details for Profilin

Target: Profilin (PFN)

Alternative Name: Profilin (PFN Products)

Synonyms: LOC406154 antibody, GB13380 antibody, profilin antibody, PFN antibody, PRF antibody

Background: Profilin (PFN1) is a ubiquitous small (12-15  kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).

Gene ID: 5216

NCBI Accession: NP_005013

UniProt: P07737