GNAI1 antibody

Catalog No. ABIN5699458

Product Details anti-GNAI1 Antibody

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Immunogen: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

human brain tissue were subjected to SDS PAGE followed by western blot with FNab03531(GNAI1 antibody) at dilution of 1:1600

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for GNAI1

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Alternative Name: GNAI1 (GNAI1 Products)

Synonyms: AU046200 antibody, Gialpha1 antibody, Gnai-1 antibody, gnai1 antibody, Gi antibody, BPGTPB antibody, zgc:63957 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting 1 antibody, G protein subunit alpha i1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 S homeolog antibody, guanine nucleotide-binding protein G(i) subunit alpha-1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 antibody, Gnai1 antibody, gnai1.S antibody, GNAI1 antibody, LOC397505 antibody, gnai1 antibody

Background: Synonyms: Background:Guanine nucleotide-binding proteins(G proteins) function as transducers downstream of G protein-coupled receptors(GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins(PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels(By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis(PubMed:17635935).

Molecular Weight: 40 kDa

Gene ID: 2770

UniProt: P63096

Pathways: G-protein mediated Events