Histone 3 antibody (H3K36me2)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K36me2
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Reactivity
- Human, Mouse, Rat, Chicken, Xenopus laevis, C. elegans, Drosophila melanogaster, Plant
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Characteristics
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Synonyms: rabbit anti-Histone H3 dimethyl Lys36 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K36me2
Background: In normal cellular conditions, H3K36Me2 represses spurious transcription, via recruitment of a deacetylase complex, Rpd3s. However, in cancer cells, overexpression of a histone demethylation enzyme (KDM8) can lead to demethylation of H3K36Me2, which then leads to inhibition of HDAC recruitment and thus erroneous transcriptional activation. Therefore, the variable role of H3K36Me2 as an epigenetic repressor or activator seems critical in cancer research. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST2H3C
- Purification
- Anti-Histone H3 [Dimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Immunogen
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Immunogen: Histone H3 [Dimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 36 of human Histone H3.2.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:500
Application Note: Anti-Histone H3 [Dimethyl Lys36] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.73 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Gene ID
- 126961
- NCBI Accession
- NP_001005464
- UniProt
- Q71DI3
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