Histone 3 antibody (H3K79me)

Catalog No. ABIN5706778

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K79me

Reactivity: Human, Mouse, Rat, Xenopus laevis, C. elegans, Drosophila melanogaster, Plant, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Characteristics:

Synonyms: rabbit anti-Histone H3 monomethyl Lys79 antibody, H3K79me1, Anti-H3 K79-Me1, Histone H3 antibodies

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular Lys79 methylations are involved in transcriptional activation, and has been found to be inversely correlated with H2B ubiquitination. Anti-Histone H3 K79-Me1 antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Purification: Anti-Histone H3 [Monomethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Immunogen:

Immunogen: Histone H3 K79-Me1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the Lys79 site of human Histone H3.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Histone H3 K79-Me1 antibody is useful in Western Blot, ChIP, Immunofluorescence, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15  kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2 μg

Western Blot Dilution: 0.5 μg/mL

IF Microscopy Dilution: 1:50 - 1:100

Comment:

Dot Blot - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Gene ID: 3020

NCBI Accession: NP_002098

UniProt: P84243