Histone 3 antibody (H3K9ac, pSer10)

Catalog No. ABIN5706780

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K9ac, pSer10

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Characteristics:

Synonyms: rabbit anti-Histone H3 Ac Lys9 pS10 antibody, Histone H3 antibodies, H3K9ac/pS10

Background: Histones of the nucleosome build chromatin and undergo various post-translational modifications proven to regulate chromatin condensation and DNA accessibility. Phosphorylations on Serine 10 has been shown to facilitate chromatin condensation by its interaction with Aurora-B kinase during mitosis and is alternatively active in cell division. Serine modification is associated with IKK-alpha and Snf1 enzymes in transcriptional activation, and is modified by MSK1 and 2 which are enzymes that react to growth factors and cellular stress in immediate-early gene activation. Lysine 9 acetylations are connected with histone deposition and transcriptional activation. Anti-Histone H3 [ac Lys9/phospho Ser10] antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Purification: Anti-Histone H3 K9Ac/pS10 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. Cross-reactivity with other sources has not been determined.

Immunogen:

Immunogen: Anti-Histone H3 [ac Lys9/phospho Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a acetylation surrounding Lysine 9 and a phosphorylation surrounding serine 10 of human Histone H3.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Histone H3 K9-Ac/pS10 antibody is useful for Western Blot, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15 kDa corresponding to Histone H3 protein by Western Blotting in NIH-3T3 histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1.0 μg/mL

IF Microscopy Dilution: 1:10

Comment:

Dot Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3