Histone 3 antibody (H3K9ac)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K9ac
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Reactivity
- Human, Mouse, Rat, Chicken, Xenopus laevis, C. elegans, Drosophila melanogaster, Plant
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Characteristics
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Synonyms: rabbit anti-Histone H3 Ac Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac
Background: Acetylation of histone H3 at the K9 residue is associated with chromosome condensation in mitotic cells. The presence of H3K9ac is correlated to H3K4 trimethylation, and their distribution is related to developmentally repressed genes in some species. It has been shown that neuron depolarization promotes intragenic histone acetylation leading toward the formation of this modification. In pituitary, dopamine receptor agonists lead toward apoptosis. However, in tumors, decreased availability and activity of these agonists, combined with decreased H3K9ac leads toward resistance to chemotherapies and cancer survival and proliferation. Nuclear receptor PPAR ' induces the enrichment of H3K9ac enrichment on downstream promoters, which has effects on transcription and ultimately nuclear receptor transactivation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST2H3C
- Purification
- Anti-Histone H3 [ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Immunogen
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Immunogen: Histone H3 [ac Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 9 of human Histone H3.2.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:100
Application Note: Anti-Histone H3 [ac Lys9] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:100
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.68 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Gene ID
- 126961
- NCBI Accession
- NP_001005464
- UniProt
- Q71DI3
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