IL1R1 antibody

Catalog No. ABIN5706822

Product Details anti-IL1R1 Antibody

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Characteristics:

Synonyms: IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, Antigen CD121a antibody, CD121A antibody, CD121a antigen antibody, D2S1473 antibody, IL 1 antibody, rabbit anti-IL-1 Receptor Type I Antibody, rabbit anti-interleukin-1 Receptor Type I Antibody

Background: IL-1 Receptor Type I is a cytokine receptor which binds interleukin 1. Anti-IL-1 Receptor Type I antibody is ideal for researchers interested in Immunology, Signal Transduction and Cancer.

Gene Name: IL1R1

Purification: This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells.   In general, this antibody also detects primate IL-1RI.  The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or ''decoy'' receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.

Immunogen:

Immunogen: IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use in ELISA,  radioimmunoassays and immunoprecipitation.  Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by ELISA against soluble (extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay. 

Neutralization Dilution: User Optimized

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 84 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL-1 Receptor Type I (IL1R1 Products)

Synonyms: CD121A antibody, D2S1473 antibody, IL-1R-alpha antibody, IL1R antibody, IL1RA antibody, P80 antibody, CD121a antibody, CD121b antibody, IL-iR antibody, Il1r-1 antibody, DER4 antibody, Fit-1 antibody, Ly84 antibody, ST2L antibody, St2 antibody, St2-rs1 antibody, T1 antibody, T1/ST2 antibody, interleukin 1 receptor type 1 antibody, interleukin 1 receptor, type I antibody, interleukin 1 receptor-like 1 antibody, IL1R1 antibody, Il1r1 antibody, Il1rl1 antibody

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints