Histone 3 antibody (H3K4me, pThr3)

Catalog No. ABIN5706875

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me, pThr3

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis, C. elegans, Drosophila melanogaster, Plant

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Characteristics:

Synonyms: rabbit anti-Histone H3 pT3 monomethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87782, MGC87783,H3pT3/K4me1

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, methylation of lysine 4 on H3 (H3 K4Me) and phosphorylation of threonine 3 (H3 pT3) are known marks of transcriptional activation and mitosis, respectively. While H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1), Haspin is the only known modifier for H3T3. Recent findings also demonstrate that pT3 can promote binding of survivin in the nucleosome. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones.

Gene Name: HIST2H3C

Purification: Anti-Histone H3 [p Thr3, Monomethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Immunogen:

Immunogen: Histone H3 [p Thr3, Monomethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 3 of human Histone H3.2.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Histone H3 K4me1/pT3 Antibody is useful for Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3