Histone 3 antibody (2meLys2 (symetric), pThr3)

Catalog No. ABIN5706882

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: 2meLys2 (symetric), pThr3

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Characteristics:

Synonyms: rabbit anti-Histone H3 pT3 Sym-dimethyl Arg2 antibody, Histone H3 pT3 dimethyl R2s, Histone H3 pT3/R2Me2s, Histone H3 antibodies

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular phosphorylation of threonine 3 (H3 pT3) is a known mark of mitosis. Recent findings also demonstrate that pT3 can promote binding of survivin in the nucleosome. Arg2 may have opposing effects to pT3. Anti-Histone H3 pT3/R2Me2s is ideal for researchers Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene Name: HIST2H3C

Purification: Anti-Histone H3 pT3/R2Me2s was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Immunogen:

Immunogen: Histone H3 pT3/R2Me2s affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT3 and R2Me2s site of human Histone H3.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Histone H3 pT3/R2Me2s antibody is useful in Western Blot, Immunocytochemistry, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15  kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1:50-1:100

Comment:

Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3