XRCC6 antibody (Ser5)

Catalog No. ABIN5960007

Product Details anti-XRCC6 Antibody

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Binding Specificity: Ser5

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: 5''-deoxyribose-5-phosphate lyase Ku70,5''-dRP lyase Ku70,70 kDa subunit of Ku antigen,ATP dependent DNA helicase 2 subunit 1,ATP dependent DNA helicase II 70 kDa subunit,ATP-dependent DNA helicase 2 subunit 1,ATP-dependent DNA helicase II 70 kDa subunit,CTC box binding factor 75 kDa subunit,CTC box-binding factor 75 kDa subunit,CTC75,CTCBF,DNA repair protein XRCC6,G22P1,Ku 70,Ku autoantigen p70 subunit,Ku autoantigen,70  kDa,Ku p70,Ku70,Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa,Kup70,Lupus Ku autoantigen protein p70,ML8,Thyroid autoantigen 70kD (Ku antigen),Thyroid autoantigen,Thyroid lupus autoantigen,Thyroid lupus autoantigen p70,Thyroid-lupus autoantigen,TLAA,X ray repair complementing defective repair in Chinese hamster cells 6,X-ray repair complementing defective repair in Chinese hamster cells 6,X-ray repair cross-complementing protein 6,XRCC 6,Xrcc6,XRCC6

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Ku-70 around the non-phosphorylation site of Ser5.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: Ku-70 (XRCC6 Products)

Synonyms: CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, 70kDa antibody, G22p1 antibody, Ku70 antibody, Kup70 antibody, X-ray repair cross complementing 6 antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody, XRCC6 antibody, Bm1_41430 antibody, xrcc6.L antibody, Xrcc6 antibody

Background: Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Molecular Weight: 70kDa

Gene ID: 2547

UniProt: P12956

Pathways: DNA Damage Repair