HIF1A antibody (AA 530-825) (Biotin)
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- Target See all HIF1A Antibodies
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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Binding Specificity
- AA 530-825
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Reactivity
- Human, Mouse, Rat, Primate
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HIF1A antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Recognizes mouse HIF-1 alpha. Species cross-reactivity: rat, human, primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples.
- Purification
- Immunoaffinity purified
- Immunogen
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A fusion protein including aa530-825 of the mouse HIF-1 alpha protein.
Type of Immunogen: Fusion protein - Top Product
- Discover our top product HIF1A Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 - 10 μg/mL), WB (1:500 - 1:2000)
Usage: Suitable for Western Blot and Immunohistochemistry. Not recommended for Immunoprecipitation. Western Blot: 1:500-1:2000. Immunohistochemistry (regular/paraffin): 5-10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, no preservatives added.
- Preservative
- Without preservative
- Handling Advice
- avoid freeze thaw cycles. Store undiluted.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- Target
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Alternative Name
- HIF1A / HIF1 Alpha (HIF1A Products)
- Synonyms
- hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody
- Background
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Name/Gene ID: HIF1A
Synonyms: HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1 - Gene ID
- 3091
- UniProt
- Q16665
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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